Coding

Part:BBa_K5249029:Design

Designed by: Jiasong Huang   Group: iGEM24_HBUT-China   (2024-10-01)


122IsPETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 789
    Illegal PstI site found at 452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
    Illegal NotI site found at 817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal XhoI site found at 555
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 789
    Illegal PstI site found at 452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 789
    Illegal PstI site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing


Source

ISPETase is an enzyme that degrades PET and is produced by the bacterium Ideonella sakaiensis

References